Subpopulation diversity of purified T cells from HIV-uninfected subjects. (A) Diversity (TCR sequences measured per 1.2 million cells) of naive CD4⁺ T cells (4N; CD3⁺CD4⁺CCR7⁺CD45RA⁺CD57⁻), central memory CD4⁺ T cells (4CM; CD3⁺CD4⁺CCR7⁺CD45RA⁻CD57⁻), and naive CD8⁺ T cells (8N; CD3⁺CD4⁺CCR7⁺CD45RA⁺CD57⁻) sort-purified from HIV-uninfected subjects. (B) Absolute diversity (TCR sequences measured per 0.4 million cells) of central memory CD8⁺ T cells (8CM; CD3⁺CD8⁺CCR7⁺CD45RA⁻CD57⁻), effector memory CD4⁺ T cells (4EM; CD3⁺CD4⁺CCR7⁻CD45RA⁺), and effector memory CD8⁺ T cells (8EM; CD3⁺CD4⁺CCR7⁻CD45RA⁺) sorted from HIV-uninfected subjects. One-way ANOVA for the 6 populations was significant (Kruskal-Wallis test, P < .0001). The 4N and 8N populations did not have a significant difference in sequences per sample (Mann-Whitney test, P = .32), but the 4CM population had significantly less diversity than 4N and 8N and significantly more diversity than 8CM (all comparisons with 2-tailed Mann-Whitney tests, P < .0001).