Hemostatic effect of mAb 2021 in hemophilia-like plasma and blood. Hemophilia-like conditions were induced by incubation of normal human plasma or blood with 100 μg/mL FVIII Ab (sheep anti–human FVIII; Hematologic Technologies Inc) for 30 minutes at room temperature. (A) dPT clot analysis with various concentrations of mAb 2021 spiked into normal human plasma (○) and into FVIII-neutralized plasma (●). Results are presented as mean ± SD (n = 3). (B-C) Clot time (B) and clot development values (C) obtained by TEG analysis when various concentrations of mAb 2021 were spiked into FVIII-neutralized human blood. Data are presented as results from individual donors (donor 1: ▴, donor 2: ■, donor 3: ●, donor 4: ▾). In the absence of mAb 2021 and anti-FVIII Ab the mean (± SD) clot time obtained in normal blood from the 4 donors was 12.4 ± 3.8 minutes and the clot development was 6.8 ± 1.2 mm × 100 × s⁻¹.