Effect of mAb 2021 and rFVIII on TF-induced fibrin clot formation in FVIII immune-depleted plasma measured by thrombin generation. Effect of mAb 2021 and rFVIII on TF-induced fibrin clot formation in FVIII immune-depleted plasma (Dade Behring GmbH) measured by thrombin generation. Coagulation was initiated by recalcification and addition of Innovin (0.12pM TF) to FVIII-depleted plasma with 150 000 platelets/μL in (A) the absence and (B) presence of 10nM mAb 2021. Thrombin generation was followed in FVIII-depleted plasma in absence of added rFVIII (a) and in FVIII-depleted plasma replenished with rFVIII: (b) 0.005 IU/mL; (c) 0.05 IU/mL; or (d) 1.0 IU/mL.