Exacerbated Cxcl12-induced chemotaxis of Cxcr4+/1013 leukocytes. (A) Cxc and Cc receptors expression on gated thymocytes (CD3+), BM B cells (CD19+) and neutrophils (SSChighGr1high), spleen B cells (B220+), and blood T (CD3+) and B (CD19+) cells from WT and Cxcr4+/1013 (+/1013) mice. Background fluorescence is shown (shaded area). (B) Migration of blood T and B cells (left) and of double-positive (DP) and single-positive (SP) CD4+ thymocytes (right) in response to Cxcl12 (with or without AMD3100), Cxcl13, Ccl21, or Ccl25. (C) Cell surface expression of Cxcr4 and Ccr9 in DP thymocytes on exposure to Cxcl12 and Ccl25, respectively. Receptor expression on thymocytes incubated in medium alone was set as 100%. Results represent the means ± SD of 3 to 5 independent experiments (B-C) or are representative of 3 to 5 independent determinations (A), (*P < .05; **P < .005 compared with WT leukocytes).
