Decreased numbers of splenic B and T cells in Cxcr4^+/1013 mice. (A) Spleen wet weight (left) and total cell number (right) were determined. (B) Spleen sections were immunostained for B220, CD3, or MOMA-1 (brown) or for B220 (green) and Thy1.2 (red). Marginal zone (mz), white pulp (wp), and red pulp (rp) are indicated. Original magnification, ×40 (B220, CD3 and MOMA-1) and ×200 (B220/Thy1.2); scale bars denote 500 μm. (C) Frequencies (mean ± SD, n = 8-12; left) and absolute numbers (right) of total spleen B cells (gated B220⁺CD3⁻) and T cells (gated B220⁻CD3⁺CD4⁺ or CD8⁺) are shown. (D-E) Absolute numbers of (D) transitional (type T1 and T2), marginal zone (MZ), and follicular (FO) B cells, and of (E) naive and effector/memory (Eff/Mem) CD4⁺ or CD8⁺ T cells, as determined in supplemental Figure 5. Results are from all the analyzed littermates (A,C-E), are from 1 representative mouse (C, left) or are representative of 3 independent determinations (B). Lines indicate the mean and each circle indicates an individual mouse (A,D-E), (*P < .05; **P < .005 compared with WT mice).