Increased numbers of LN T cells in Cxcr4^+/1013 mice. (A-B) The wet weights (mean ± SD, n = 6-10; A) of inguinal (i) and axillary (a) LNs were determined as well as (B) total cell numbers, frequencies (left), and absolute numbers (right) of total B and T cells. (C) aLN sections were immunostained for B220, CD3 (brown), or for B220 (green) and Thy1.2 (red). Cortex (c), paracortex (pc), and medullary (m) areas are indicated. Original magnification, ×40 (B220 and CD3) and ×200 (B220/Thy1.2); scale bars denote 500 μm. (D) Absolute numbers (mean ± SD) of naive and effector/memory (Eff/Mem) CD4⁺ or CD8⁺ T cells in peripheral LNs are shown. Results are from all the analyzed littermates (A,B,D), are from 1 representative mouse (B, left), or are representative of 3 independent determinations (C), (*P < .05; **P < .005 compared with WT mice).