TF- and gC-restricted HSV1 panel. An equal number of purified virus particles (7.5 × 10¹⁰) were separated electrophoretically through a 10% polyacrylamide gel, followed by Western blot analysis. The presence of TF (A) and HSV1 gC (B) were detected by Western blot. HSV1 major capsid protein (MCP) was used as a virus particle loading control (co-blotted in panel A). Protein Ag identification was consistent with molecular weight markers (not shown).