Combined TF and gC on HSV1 optimally enhance FXa/FVIIa-mediated infection. HUVECs in SFM were inoculated with HSV1 (4.5 × 10⁵ vp/mL: ■, TF⁺/gC⁺; ▴, TF⁺/gC⁻; ●, TF⁻/gC⁺; ▾, TF⁻/gC⁻) at the indicated concentration of thrombin (A), FXa (B), FVIIa (C), and FXa at a constant concentration of FVIIa (0.5nM; D). The cells were stained 24 hours after infection and the amount of infection was determined. The data were corrected for the number of plaques detected in the absence of added protease. For panel A, n = 4; B, n = 4; C, n = 6; and D, n = 6. Data are ± SEM.