Viral TF is involved in FXa- and FVIIa-mediated HSV1 infection. HUVECs were incubated with a constant amount of TF⁺/gC⁺ (A) or TF⁻/gC⁺ (B) HSV1 (4.5 × 10⁵ vp/mL) and thrombin (10nM), FXa (1nM), FVIIa (2.5nM), or FXa/FVIIa (1nM/0.5nM) with murine IgG (No Enzyme, 55nM), mIgG plus enzyme (mIgG), or enzyme plus the indicated anti-TF (55nM), NAPc2 (50nM) or hirudin (10 U/mL). The data were corrected for the amount of infection detected without added protease (n = 4; data are ± SEM). *P ≤ .05 compared with control IgG plus enzyme.