Antibodies against VWF inhibit PMN recruitment to the inflamed peritoneum. (A) Mice were treated with either control rabbit IgG (Co-IgG), anti-P- and L-selectin antibodies, or rabbit anti-VWF antibodies (5 mice per group). Upon antibody injection peritonitis was induced by i.p. application of thioglycollate. After 2 hours, PMNs were collected and counted. (B) Mice were injected with Co-IgG or anti-VWF antibodies (4 mice per group) as indicated. Peritonitis was induced as mentioned in panel A, and transmigrated PMNs were analyzed after 2 hours (black bars), 4 hours (gray bars), and 6 hours (white bars). (C) Mice were either treated with Co-IgG F(ab′)2, anti-P- and L-selectin antibody, or anti-VWF F(ab′)2 (3 mice per group). Peritonitis was induced and analyzed as indicated in panel A. (D) Peritonitis assays were performed as indicated in panel A with an additional group where the anti-VWF antibody was depleted of VWF-reactivity with immobilized recombinant VWF protein (4 mice per group). Error bars show SEM values. *P ≤ .05; **P ≤ .01; ***P ≤ .001. Representative analyses from 10 experiments (A), 2 experiments (B-C), and 1 experiment (D) are shown.
