PMN recruitment is independent of platelet-derived VWF and thioglycollate stimulates luminal deposition of endothelial VWF. (A) Mice were lethally irradiated and reconstituted either with wt or VWF^-/- donor bone marrow. Peritonitis was induced with thioglycollate. After 2 hours, PMNs were collected and counted in the different groups (n ≥ 5). Error bars show SEM. ***P ≤ .001. (B) Wild-type (WT), VWF^−/− (KO), or wild-type mice transplanted with VWF^−/− bone marrow (KO transplanted) were injected intraperitoneally with either thioglycollate (stimulated) or PBS (unstimulated). After 1.5 hours, primary labeled anti-PECAM antibodies and nonlabeled anti-VWF antibodies were intravenously injected. After 30 minutes, the vascular system was perfused with PBS to remove unbound antibodies, mesenteries were dissected and stained as whole mounts with a labeled secondary antibody for VWF. Scale bar = 20 μm.