Antibodies against VWF and platelet-depeletion reduce blood vessel permeability in the inflamed peritoneum. (A) Mice were intravenously injected with Evans blue dye and either control IgG (black bars) or anti-VWF antibody (white bars) before they were treated with PBS or thioglycollate (thio). Evans Blue was removed from the peritoneum by lavage 1 hour later. One representative experiment out of 3 is shown (n = 3-5). (B) Mice received an intravenous injection of either Co-IgG or a platelet-depleting antibody. After 24 hours, mice were injected intravenously with Evans blue dye then intraperitonally with thioglycollate. After 1 hour, Evans blue dye was quantified from peritoneal lavage (n ≥ 9). (C) Mice were intravenously injected with Evans blue dye and either isotype control Ab or Gr-1 Ab for PMN depletion before inflammation was induced with thioglycollate. Permeability was determined by optical density 620 measurement of the peritoneal lavage. Error bars show SEM. *P ≤ .05; **P ≤ .01.