AIM1 is methylated in the DERL2 HSTL cell line. (A) Real-time PCR quantification of AIM1 was evaluated innormal γδ T cells, HSTL tissues, HSTL cells, DERL2 cells, PTCL-NOS, and NKTCL. The results are expressed as the relative fold change compared with normal γδ T cells sorted from the spleen. Each sample was normalized to GAPDH. (B) Localization of CpG islands (CpG1 and CpG2) in the AIM1 promoter and schematic representation of the sequenced genomic fragments including all CpG sites (vertical bars). Approximate spacing of the CpG dinucleotides is shown for the 44 CpGs of island 1 and the 119 CpGs of island 2 (top). Ten representative clones from DERL2 cells are presented, only 43 CpGs of island 2 were represented because of unmethylation of the first 76 CpGs (left). Example of methylated cytosines after bisulfite-converted genomic DNA treatment (right). (C) Real-time PCR quantification of AIM1 was evaluated in DERL2 cells after 96 hours of 10 or 40μM decitabine treatment (every 24 hours) with or without 500nM TSA treatment for the last 24 hours. (D) DERL2 cells were cultured with or without 10 or 40μM decitabine for 96 hours (decitabine was added every 24 hours). Cellular apoptosis was analyzed using 7AAD staining.