Effects of c-myb silencing on cell-cycle distribution and clonogenic activity of CD34⁺ cells. (A) Western blot analysis of c-myb expression 24 hours after nucleofection. (B) Results of statistical analysis on the percentage of cells in the different cell-cycle phases performed by PI staining 24 hours after nucleofection (mean ± 2 SEM; n = 8). (C) Methylcellulose clonogenic assay results (mean ± 2 SEM; n = 8). Values are reported as percentages. (D-E) Megakaryocyte clonogenic assay results (mean ± 2 SEM; n = 4) in terms of megakaryocyte colony number (D) and size (E). Values are reported as number of megakaryocyte colonies for 1000 plated cells. Error bars in the graphs represent SD. *P ≤ .05, **P ≤ .01, and ***P ≤ .001 vs both MOCK and NegCTR samples. n = number of experiments.