c-myb silencing effects on CD34⁺ stem/progenitor cells differentiation. (A) Flow cytometric analysis of CD14 and CD163 (day 11 after nucleofection), GPA and CD41 (day 7 after nucleofection) markers after liquid multilineage culture (mean ± 2 SEM; n = 8). Error bars in the graphs represent SD. ***P ≤ .001 in MYBsiRNA versus MOCK and NegCTR. (B) Flow cytometric analysis of (i) GPA, CD41, (ii) CD15 and CD14 markers after liquid unilineage (i) erythroid, megakaryocyte, (ii) granulocyte and mono-macrophage culture, respectively (n = 3). For each marker, flow cytometry data at 5, 9, and 13 days after nucleofection are reported. Error bars represent SD. *P ≤ .05, **P ≤ .01, and ***P ≤ .001 in MYBsiRNA versus MOCK and NegCTR. (C) Table summarizing flow cytometry data for unilineage cultures (mean ± 2 SEM; n = 3). (D-E) Morphological analysis of May-Grunwald-Giemsa–stained cytospins after both liquid multilineage culture (day 11; D) and liquid unilineage (E) erythroid (day 7, i-iii), megakaryocyte (day 7, iv-vi), granulocyte (day 11, vii-ix) and mono-macrophage (day 11, x-xii) culture after nucleofection. The images were captured by Axioskop 40 microscope, by means of AxioCam HRc Digital Camera and Axiovision software 3.1 (all Carl Zeiss MicroImaging Inc). The images were then processed with Adobe Photoshop 7.0 software. Original magnification ×630. n = number of experiments.