c-myb silencing effects on CD34⁺ cells gene expression profile. Microarrays data of (A) cell-cycle–related, (B) erythroid, granulocyte, (C) mono-macrophage, and (D) megakaryocyte genes. * indicates genes already reported as c-myb targets. (E) Microarrays data validation by qRT-PCR of a subset of transcripts (mean ± 2 SEM; n = 3). Error bars represent SD. *P ≤ .05 and **P ≤ .01 in MYBsiRNA versus MOCK and NegCTR. n = number of experiments. (F-G) Western blot detection of KLF1 (F) and LMO2 (G) expression levels 24 hours after nucleofection.