c-myb silencing effects on CD14⁻ myeloblasts gene expression profile and validation of PDCD4 as c-myb direct target gene. Microarrays data of (A) mono-macrophage, (B) cell-cycle–related, and (C) granulocyte genes. * indicates genes already reported as c-myb targets. (D) PDCD4 promoter-specific PCR performed in a representative ChIP experiment. CTSG promoter-specific PCR is shown as a positive control for immunoprecipitation. ChIP and PCRs were performed in triplicate. (E) c-myb transactivation of PDCD4 promoter-driven luciferase expression. The amounts (in micrograms) of cotransfected plasmids are reported. Luciferase levels (mean ± 2 SEM;n = 3) are normalized by setting the empty pXP1/pCMV6XL5 vectors-transfected sample as = 1. Error bars represent SD. *P ≤ .05 and **P ≤ .01 vs pXP1PDCD4-921+empty pCMV6XL5-transfected sample. n = number of experiments.