Treg reconstitution in CD8⁺ young TIL trial. (A-B) Percentages and cell counts of Tregs in PBMCs from CD8⁺ young patients are shown. (C) Nonparametric correlation between doses of IL-2 administrated and percentages or absolute cell count of Treg from week-1 and week-4 PBMCs in CD8⁺ young TIL trial. Spearman r and P values are shown. (D) Percentages of Tregs in CD3⁺ T cells and Treg counts of nonresponders (NR) and responders (R) in the CD8⁺ young TIL trial are shown. (E-F) Nonparametric analysis was used to compare the percentages and absolute cell counts of CD4⁺ Tregs at week 1 in the peripheral blood of nonresponders and responders from the 4 NMA TIL trials. Horizontal bars represent the median values.