Comprehensive analysis of DNA methylation and gene expression. (A) Unsupervised hierarchical clustering based on differentially methylated CpGs is shown on the dendrogram. The accompanying heatmap shows the methylation status across 5001 differentially methylated CpGs. In the heatmap, red indicates a CpG methylation more than 50%, and green less than 50%. The methylation status in hypo SS DMRs (B) or hyper SS DMRs (C) was shown in the heatmap. (D) Unsupervised hierarchical clustering based on global gene expression data are shown on the dendrogram. The accompanying heatmap shows the normalized log2 transformed expression values (Z-scores) for each probe. In the heatmap, red indicates expression more than mean, and green less than mean.