Hematopoietic differentiation of CML-iPSCs. CML-iPSCs were differentiated on the 10T1/2 cells. On day 7 (A), iPSCs began to mount. On day 14 of culture (B: left panel), inflated sac-like structures appeared. These sac-like structures contained the round hematopoietic cells (B: right panel: higher magnification). (C) These hematopoietic cells expressed immature marker CD34 and CD45. (D) CFC activity was estimated using 1 × 10⁴ 3CD34⁺ CD45⁺cells. Erythroid colonies (black bars), granulocyte-monocyte (GM) colonies (white bars), and mixed GM colonies with erythtoid cells (mix; gray bars) were plotted.