Figure 4
![Figure 4. Splenocytes from mice receiving anti–IL-21 mAb have decreased numbers of cells secreting IFN-[gamma], IL-17, and GrB. Spleens were isolated on day 10 and day 20 from NOD/SCID/γc−/− mice receiving human PBMCs (30 × 106 cells) that were treated with nothing, anti–IL-21 mAb, or isotype control antibody, and splenocytes were stimulated with phorbol myristate acetate and ionomycin and assayed using intracellular cytokine staining. Average percentage (± SEM) of cells secreting IFN-γ (A-B), IL-4 (C-D), IL-17 (E-F), and GrB (G-H) on day 10 (A,C,E,G) or day 20 (B,D,F,H). n = 4 mice for each time point (day 10 and day 20) and for each treatment group.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/119/2/10.1182_blood-2011-07-368027/5/zh89991184110004.jpeg?Expires=1735828880&Signature=ExSTyCUvScADJsi~vPcuQyKgKuYItL4VtsoS1Ey1V4FkVpPpYUez~hrTgDVyyBqzDZ4BiUM2Cjt6ShlI1zhrA4AaCUzo4O8-AW8dXWH~0wtHWrU~8AiAgTZOfWhN9efkkBZF8pwXDxU2jDHpJSDBuKRND0VtHtdmKXTBA-4B~LhvAYSQB3AI42ddgXqmus9vTxvI5mUWPwtzfss0iafaR8a1AARb2nqopMQWav5jUwHZIcN2t5CvVRT3N-0httmZEjDTQYebayD0J5W~Ok~1Jf6JiF5XaYOe-5fVeveK7VPYGw2EOmk4mlr9PDwqLCl1cEyaaKe2D3Zhq3i0aNWcoA__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Splenocytes from mice receiving anti–IL-21 mAb have decreased numbers of cells secreting IFN-[gamma], IL-17, and GrB. Spleens were isolated on day 10 and day 20 from NOD/SCID/γc−/− mice receiving human PBMCs (30 × 106 cells) that were treated with nothing, anti–IL-21 mAb, or isotype control antibody, and splenocytes were stimulated with phorbol myristate acetate and ionomycin and assayed using intracellular cytokine staining. Average percentage (± SEM) of cells secreting IFN-γ (A-B), IL-4 (C-D), IL-17 (E-F), and GrB (G-H) on day 10 (A,C,E,G) or day 20 (B,D,F,H). n = 4 mice for each time point (day 10 and day 20) and for each treatment group.
