Mutant alleles of Rhbdf2 are noncomplementary and cause a specific block in TNFα secretion. Thioglycollate-elicited peritoneal cells were isolated from mice of the indicated genotypes, and cultured in the presence of 200 pg/mL MALP-2, 1 ng/mL LPS, or media alone (-) for 4 hours. TNFα (A) and IL-6 (B) was then measured in the culture supernatant.