FVIII and VWF levels in macLRP1⁺ and macLRP1⁻ mice. Plasma samples were collected from age- and sex-matched macLRP1⁺ mice (n = 42; open circles) and macLRP1⁻ mice (n = 39; closed circles) and analyzed for FVIII (A) and VWF (B). Individual FVIII levels also were plotted versus individual VWF levels (D). For each mouse, the ratio FVIII over VWF was calculated. The average ratio (± SD) for macLRP1⁺ and macLRP1⁻ is provided in panel E. In another group of macLRP1⁺ (n = 19; open circles) and macLRP1⁻ mice (n = 26; closed circles), the time to arrest of bleeding in a tail-clip bleeding model (see “Bleeding time”; ***P < .0001, **P < .001, *P < .05) was determined (C).