Effect of RAP expression on FVIII and VWF levels in macLRP1⁺ and macLRP1⁻ mice. Plasma samples of MacLRP1⁺ mice (open symbols; n = 10) and macLRP1⁻ (closed symbols; n = 10) were taken 5 days before and 4 days after hydrodynamic gene transfer with pLIVE-RAP. Samples were analyzed for FVIII activity (squares) and VWF antigen (circles) levels. Data represent mean ± SD. FVIII and VWF levels before and after treatment were assessed for statistical significance using a paired t test. A statistically significant increase in FVIII activity was found in macLRP⁺ and macLRP1⁻ mice, whereas for VWF a statistically significant increase was found in macLRP⁺ but not macLRP⁻ mice.