Figure 2
Analysis of interaction of the (β15-66)2 fragment and fibrin with the LDLR family members, VLDLR and LRP, by ELISA. Increasing concentrations of sVLDLR (●) or LRP (○) were incubated with the microtiter wells coated with the (β15-66)2 fragment (A) or fibrin (B), and the bound receptors were detected with the specific mAbs as described in “Solid-phage binding assay.” The curves are representative of 2 independent experiments; error bars represent the SD of triplicate determinations. Binding of sVLDLR at 10nM to immobilized (β15-66)2 in the absence and presence of 20nM RAP is shown in panel A inset. Binding of sVLDLR at 10nM to immobilized fibrin in the absence and presence of 20nM RAP or 10μM (β15-66)2 is shown in panel B inset.
