Figure 3
Analysis of interaction of the (β15-66)2 fragment with the LDLR family members, VLDLR and LRP, by SPR. (A) The (β15-66)2 fragment at 1μM was added to immobilized sVLDLR or LRP, and its association/dissociation was monitored in real time. (B) The (β15-66)2 fragment at increasing concentrations, 0.5, 1, 2.5, 5, and 10nM, was added to immobilized sVLDLR, and its association/dissociation was monitored in real time (solid curves). The dotted curves represent the best fit of the data with the use of the kinetic analysis. The curves in panels A and B are representative of 2 and 4 independent experiments, respectively. All experiments were performed in HBS-P buffer containing 1mM CaCl2.
