![Figure 5. VWF is required for VSMC coverage in developmental angiogenesis. (A) Representative images of retinas undergoing early postnatal retinal developmental angiogenesis at 3 different time points: P3, P5, and P7. Retinas were immunostained with CD31 (red) to label the endothelium and αSMA to label VSMCs (green). (B) VWF KO mice have significantly less VSMC coverage compared with age-matched controls, measured along each artery as a percentage of the outgrowth of the vascular plexus (dotted lines). Arterial coverage is reduced 37% at P3, 21% at P5, and 17% at P7. Values represent means ± SEM. C57BL/6 (n = 12 [P3], n = 23 [P5], n = 19 [P7]), VWF KO (n = 14 [P3], n = 25 [P5], n = 14 [P7]), where n is the number of retinas analyzed. P = 9.3 × 10−4 (P3), P = 1.7 × 10−9 (P5), P = 7.3 × 10−3 (P7). Scale bars: 150 μm (P3), 300 μm (P5), and 600 μm (P7).](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/119/9/10.1182_blood-2011-04-348706/4/zh89991185040005.jpeg?Expires=1742856549&Signature=dCAm8ceXAXrRw~84-Zzz8egNkPa~Sp9vJIE9jpvHDh8rKmUQsYMTVleuOFIjfNAXXCujcb5xFWmJQ9PGDjxpIgPJeohejkoh8jzuxEJ9fDEw3kaEvv4kS1lM7dNmyheWhdbveoRYMHOnEOTBlH7A627g-9oNYAcpmk4cMpUXq41yH8GfD-D4PTLRSrjAH-ZlvQfjb-2A1IPu3-yzkGcgRycHOlLetc2LbY-Wia0cOnlIAPckPCF-vNMo7KQWY01sEcHXeXwcKIgU26DIBj9--JvGfkUgGveSN~uIXJgDczze~cKBs2DCYmNJuINs1yIwHtJHWxGgoz3SBovPbfRRQg__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
VWF is required for VSMC coverage in developmental angiogenesis. (A) Representative images of retinas undergoing early postnatal retinal developmental angiogenesis at 3 different time points: P3, P5, and P7. Retinas were immunostained with CD31 (red) to label the endothelium and αSMA to label VSMCs (green). (B) VWF KO mice have significantly less VSMC coverage compared with age-matched controls, measured along each artery as a percentage of the outgrowth of the vascular plexus (dotted lines). Arterial coverage is reduced 37% at P3, 21% at P5, and 17% at P7. Values represent means ± SEM. C57BL/6 (n = 12 [P3], n = 23 [P5], n = 19 [P7]), VWF KO (n = 14 [P3], n = 25 [P5], n = 14 [P7]), where n is the number of retinas analyzed. P = 9.3 × 10−4 (P3), P = 1.7 × 10−9 (P5), P = 7.3 × 10−3 (P7). Scale bars: 150 μm (P3), 300 μm (P5), and 600 μm (P7).
