IRF-8 drives DC commitment in a cell-intrinsic manner. Competitive mixed BM chimeras were carried out by injecting 2 × 106 B6.SJL (CD45.1+) wild-type whole BM cells with Irf8−/− or wild-type (CD45.2+) BM that were matched for HSC numbers into 800 cGy-irradiated (C57BL/6 × B6.SJL) F1 recipients (CD45.1+CD45.2+). Seven weeks after transplantation, BM and spleens were harvested, and progenitor, DC, and neutrophil development was analyzed by flow cytometry. (A,C) Representative plots showing CD45.2 and CD45.1 chimerism in one representative mouse. Graphs show (B) chimerism of common myeloid progenitor (CMPs), CDPs, and GMPs or (D) splenic CD8α− DC and BM neutrophils from wild-type (top) or Irf8−/− (bottom) reconstituted mice. Each point represents 1 mouse, with lines connecting values in a single mouse. Data are pooled from 3 experiments of 1-4 mice per group. Data for all 9 mice in each group are shown; **P < .05 based on paired students 2-tailed t test.
