Lnk inhibits FLT3-WT/ITD/TKD–driven proliferation of 32D cells. 32D cells stably expressing FLT3-WT (A), FLT3-TKD (B), or FLT3-ITD (C) were infected with either retroviral empty vector (EV), wild-type Lnk (WT), R364E mutant (RE), or E208Q mutant (EQ) coexpressing GFP. GFP⁺ cells were measured daily by flow cytometry and calculated as percentage of GFP⁺ cells present at 48 hours after infection. In 32D/FLT3-ITD cells, assays were also performed in the presence of IL3. Data represent the means ± SD of 3 independent experiments.