Influence of CD1d-truncated cytoplasmic tail on hematopoiesis. Effects of anti-CD1d Abs on colony formation by BM CFU-GM from (WT) control, CD1d^−/−, and CD1d-truncated cytoplasmic tail domain knock-in mice stimulated with PWMSCM, SCF, and hemin (A) and comparative hematopoiesis in the BM (B) and spleen (C) of WT control, CD1d^−/−, and truncated cytoplasmic tail CD1d knock-in mice. Results for panel A are for 1 representative of 2 similar experiments expressed as means ± SEM. a indicates enhancement of colony formation by different CD1d Abs compared with their isotype control Abs (P < .001); all other comparisons are not significant (P > .05). Results for panels B (BM) and C (spleen) shown are means ± SEM of 3 mice/group for absolute numbers (i) and cycling (ii) of hematopoietic progenitor cells (CFU-GM, BFU-E, and CFU-GEMM). In panels B and C, P values are compared with WT controls. NS indicates not significant (P > .05). (D) Influence of the combinations of GM-CSF or IL-3 with either FL or SCF on colony formation by BM CFU-GM from control, CD1d^−/−, or CD1d truncated cytoplasmic domain knock-in mice. (E) Influence of suppressive cytokines/chemokines on colony formation by BM CFU-GM from control, CD1d^−/−, or CD1d-truncated cytoplasmic domain knock-in mice. For panel D, a indicates synergistic stimulation of colony formation by the combination of murine GM-CSF (10 ng/mL) or IL-3 (10 ng/mL) with either human FL (100 ng/mL) or murine SCF (50 ng/mL); P ≤ .05. For panel E, a indicates significant suppression of colony formation. Results for panels D and E are for 1 representative of 2 similar experiments ± SEM.