High Bcl-2 expression correlates with sensitivity to ABT-263. The level of expression of 10 Bcl-2 family members in 39 NHL cell lines was assayed by microarray. (A) The heat-map represents the mRNA expression levels of Bcl-2 family genes. Red represents high expression, green low expression. ABT-263-DX is the linear predictor of cell line resistance against ABT-263 (see “Microarray and predictive model”). The bottom panel displays ABT-263 IC₅₀ values, presence of the t(14:18) translocation (IGH:BCL2) or amplification of Bcl-2 (> 3 copies) and ABC subtype classification. (B) The coefficients of the Bcl-2 family genes have been represented as a linear predictor. The value of each coefficient represents its relative contribution to the predictive signature. (C) In patients treated with R-CHOP and CHOP,³³  a low ABT-263-DX value correlated with poor prognosis. The corresponding hazard ratios and P values were calculated using Cox regressing model fitting the ABT-263-DX value as a continuous variable.