YS- and P-Sp–derived DN T cells can differentiate into distinct T-cell subsets in vivo. Cells from the recipient spleen (A) and liver (B) were analyzed for the presence of mature T-cell populations 2 weeks after transplantation. Memory (CD62L^lowCD44^high) T cells were analyzed 14 weeks after injection (A right panels, dot plot is CD45.2⁺CD4⁺ gated). Regulatory (CD4⁺FoxP3⁺) T cells, naive (CD4⁺CD62L^highCD44^low) T cells, and memory (CD4⁺CD62L^lowCD44^high) T cells were detected in the recipient spleen (A), and γδT (TCRγδ⁺CD3⁺), and α-Gal–loaded CD1d tetramer⁺ (αGC/CD1d tet⁺) CD3⁺ NKT cells were detected in the recipient liver (B). (C) TCRβ+ or TCRγδ⁺CD8⁺ intraepithelial T cells were also detected in the intestine of a mouse that received a transplant with P-Sp–derived T cells. ND indicates not determined for YS-derived cells. The representative FACS dot plots are depicted. The number of mice that received a transplant is detailed in Table 1. (D) The TCRVβ repertoires of YS-derived (left) and P-Sp–derived (right) CD3⁺ T cells engrafted in the recipient spleen are depicted (each n = 3).