VE-cadherin down-regulates N-cadherin expression and junctional localization in ECs. (A) Immunoblots of VE-cadherin, N-cadherin and α-tubulin (loading control) from VEC null and VEC positive cells. The experiment reported has been performed 3 times with comparable results. (B) qRT-PCR analysis of VE-cadherin and N-cadherin expression in VEC null and VEC positive cells. The means ± SD are graphed (n = 3; *P < .02, **P < .01). (C) Representative images of VE-cadherin and N-cadherin expression and localization in VEC null and VEC positive cells. Scale bar: 20 μm; arrows: cell-to-cell junctions. (D) Representative images of VE-cadherin +/+ and −/− allantois assays. Allantoises were stained for PECAM-1 (red) and N-cadherin (green) and their colocalization (yellow) was analyzed by confocal microscopy. Scale bar: 10 μm. (E) qRT-PCR analysis of VE-cadherin and N-cadherin expression in freshly isolated ECs obtained from lungs of VE-cadherin +/+ and ± adult mice.⁶  The means ± SD are graphed (n = 3; *P < .02). (F) Representative images of VE-cadherin +/+ and ± aorta from adult mice. The juctional localization (arrows) of VE-cadherin (green) and N-cadherin (red) was analyzed by confocal microscopy. Scale bar: 25 μm.