N- and VE-cadherin inhibit EC proliferation and survival but exert opposite effects on EC migration. (A) qRT-PCR analysis of N-cadherin and VE-cadherin expression in N, VE, and N+VE cells. The means ± SD are graphed (n = 3; *P < .05, **P < .02, ***P < .01). (B) Immunoblots of VE-cadherin and N-cadherin protein expression in N, VE, and N+VE cells. The experiment reported has been performed 3 times with comparable results. α-Tubulin is the loading control. (C) Immunoblots and quantification of total cadherin protein expression in N, VE, and N+VE cells. Columns are means ± SEM of 3 indipendent experiments, α-tubulin was used as internal loading control (*P < .05, **P < .02). (D) Immunoblots and quantification of cleaved caspase-3 from N, VE, and N+VE cells. α-Tubulin is the loading control. Columns are means ± SEM, (n = 3; *P < .05, **P < .02). (E) Quantification of the proliferation rate of N, VE and N+VE cells. The means ± SD are graphed (n = 8; *P < .05, **P < .02, ***P < .01). (F) Quantification of the migration rate of N, VE, and N+VE cells. The means ± SD are graphed (n = 6; **P < .02).