American Society of Hematology

Figure 2

$Figure 2. Global distribution of H3K27 acetylation during macrophage differentiation. (A) H3K27ac ChIP-seq tag counts for peak regions are compared between monocytes and macrophages in a density plot. The colors represent the relative density of peaks in each location within the density plot. (B) Genomic distribution of total and cell stage–specific (at least 4-fold different) H3K27ac marked regions relative to RefSeq genes. (C) Box plots show the distribution of mRNA expression levels (HSC, CD34+ HSCs; MO, monocytes; MAC, macrophages; and lymphoid cell types as indicated) for RefSeq genes adjacent to differentiation stage-specific H3K27ac peak regions, as described in Figure 1. Significance of pairwise comparisons of mRNA expression levels for the indicated cell types are indicated (***P < .001 by Student t test, paired, 2-sided). (D-E) De novo–extracted sequence motifs associated with differentiation stage–specific H3K27ac peak regions. Motifs were assigned to transcription factors or transcription factor families based on similarity with known motif matrices. In addition, the fraction of H3K27ac regions (1 kb) containing at least 1 motif instance, the expected frequency of the motif in random sequences (in parentheses), and P values (hypergeometric) for the overrepresentation of each motif are given.$

Global distribution of H3K27 acetylation during macrophage differentiation. (A) H3K27ac ChIP-seq tag counts for peak regions are compared between monocytes and macrophages in a density plot. The colors represent the relative density of peaks in each location within the density plot. (B) Genomic distribution of total and cell stage–specific (at least 4-fold different) H3K27ac marked regions relative to RefSeq genes. (C) Box plots show the distribution of mRNA expression levels (HSC, CD34⁺ HSCs; MO, monocytes; MAC, macrophages; and lymphoid cell types as indicated) for RefSeq genes adjacent to differentiation stage-specific H3K27ac peak regions, as described in Figure 1. Significance of pairwise comparisons of mRNA expression levels for the indicated cell types are indicated (***P < .001 by Student t test, paired, 2-sided). (D-E) De novo–extracted sequence motifs associated with differentiation stage–specific H3K27ac peak regions. Motifs were assigned to transcription factors or transcription factor families based on similarity with known motif matrices. In addition, the fraction of H3K27ac regions (1 kb) containing at least 1 motif instance, the expected frequency of the motif in random sequences (in parentheses), and P values (hypergeometric) for the overrepresentation of each motif are given.

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