Figure 3
Figure 3. Bacterial mutants with progressively truncated LPS backbones show increasing PF4 binding activity. S enterica sv Typhimurium wild-type strain SL3770 waa+, the isogenic LPS mutants SL3749 waaL446, SL3750 waaJ417, SL3748 waaI432, SL3769 waaG471, SL3789 waaF511, and SL1102 hldE543 (A), the E coli K-12 wild-type strain BW30270, and the LPS mutants KPM53 (ΔwaaC) and KPM121 (ΔwaaA; B) were incubated with biotinylated PF4 (20 μg/mL). PF4 binding was detected with peridinin chlorophyll protein-Cy5.5 conjugated streptavidin using flow cytometry and expressed as geometric mean fluorescence intensity (GMFI) multiplied by the percentage of labeled bacteria. Data represent mean ± SD of at least 3 independent experiments (S enterica sv Typhimurium wild-type and mutants, n = 3; E coli BW30270, n = 5; E coli KPM53, n = 5; and E coli KPM121, n = 3). **P < .01 versus wild-type. ***P < .001 versus wild-type.

Bacterial mutants with progressively truncated LPS backbones show increasing PF4 binding activity.S enterica sv Typhimurium wild-type strain SL3770 waa+, the isogenic LPS mutants SL3749 waaL446, SL3750 waaJ417, SL3748 waaI432, SL3769 waaG471, SL3789 waaF511, and SL1102 hldE543 (A), the E coli K-12 wild-type strain BW30270, and the LPS mutants KPM53 (ΔwaaC) and KPM121 (ΔwaaA; B) were incubated with biotinylated PF4 (20 μg/mL). PF4 binding was detected with peridinin chlorophyll protein-Cy5.5 conjugated streptavidin using flow cytometry and expressed as geometric mean fluorescence intensity (GMFI) multiplied by the percentage of labeled bacteria. Data represent mean ± SD of at least 3 independent experiments (S enterica sv Typhimurium wild-type and mutants, n = 3; E coli BW30270, n = 5; E coli KPM53, n = 5; and E coli KPM121, n = 3). **P < .01 versus wild-type. ***P < .001 versus wild-type.

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