Effect of Triton on MP flow cytometry analysis. (A) Representative experiment using plasma from a patient with ACS: PMP, RBC-MPs, and EMP levels were assessed using specific markers, either in absence (top panels; baseline) or presence (bottom panels) of Triton. Labeling with their respective isotype is not shown. MP number dramatically decreases after Triton lysis. (B) Plasma MP levels in ACS patients (n = 10) before and after Triton lysis. Data are expressed as mean ± SEM. AnnV+ indicates annexin V+ MPs (*P < .01; Wilcoxon test).