CD8⁻ DCs express higher levels of costimulatory and coinhibitory molecules in GVHD. (A) cDCs were stained for costimulatory/coinhibitory molecules and analyzed by flow cytometry. Data are representative of 2 replicate experiments; n = 5/group in each. *P = .05 to .01. **P = .01 to .001. (B) DCs were sort-purified on day 10 after transplantation and incubated with OVA-AlexaFluor-488. Fluorescence data reflect soluble antigen uptake. (C) Sorted DCs (day 10) were incubated for 1 hour with DQ-OVA and uptake and processing assessed by level of fluorescence after 20, 40, and 60 minutes. (D) Fluorescent beads were injected intravenously at day 10 after transplantation. Geometric mean fluorescence intensity is shown; n = 5/group. *P = .05 to .01. **P = .01 to .001.