LANA-specific CD4⁺ T-cell recognition of LCLs expressing LANA antigens. (A) IFN-γ production by GSP- or EYR-specific T cells incubated with G48 LCLs or B1 LCLs, respectively, fed either an enriched LANA or control (Cont) protein preparation. (B) Diagram of recombinant plasmids that expressed the LANA constructs and GFP reporter under the control of the tetracycline-responsive promoter. (C) Western blot analysis of recombinant LANA expressed from transfected G48 or B1 LCLs treated with dox. Blots were probed with a hemagglutinin (HA)–specific antibody to detect recombinant LANA. (D) IFN-γ secretion from GSP- or EYR-specific T cells incubated with G48 or B1 LCLs, respectively, either sensitized with 5μM cognate peptide or induced to express recombinant LANA by dox treatment.