LANA-specific CD4+ T cell recognition of PELs. (A) IFN-γ production by EYR- or GSP-specific T cells incubated with VG-1 or JSC-1 PELs, respectively, fed either an enriched LANA protein or a control protein preparation. (B) Western blot analysis of endogenous and recombinant LANA expression from transfected VG-1 or JSC-1 PELs treated with dox. Blots were probed with a LANA-specific antibody. (C) IFN-γ secretion from EYR- or GSP-specific T cells incubated with VG-1 or JSC-1 PELs, respectively, either sensitized with 5μM cognate peptide or induced to express recombinant LANA by dox treatment. (D) HLA DQ6-expressing PELs VG-1 (left) or BCBL-1 (right) were incubated with HLA DQ6-restricted T cells specific for EYR, PAF, WGD, or LAP/LRS, and then IFN-γ release was quantified.