LANA-specific CD4⁺ T-cell recognition of CIITA-transduced JSC-1, BC-1, and VG-1 PELs or unmanipulated BCBL-1 fed enriched LANA-protein. CIITA-transduced JSC-1, BC-1, or VG-1 PELs or the unmanipulated BCBL-1 PELs were fed enriched LANA protein or a control protein preparation for 18 hours before being washed and incubated with the appropriate HLA-matched T-cell clone, and then IFN-γ secretion was measured. (A-B) Protein-fed JSC-1– and BC-1–transduced PELs, respectively, assayed against the HLA DQ7-restricted GSP-specific clone. Protein-fed VG-1–transduced PELs assayed against HLA DQ6-restricted LAP/LRS-specific clones (C) or HLA DP1-restricted LAP-specific clones (D). (E) Protein-fed BCBL-1 cells assayed against the HLA DQ6-restricted EYR-specific clone.