Vav3 modulates Bcl-2 family for p190-BCR-ABL⁺ leukemic cell survival. (A) Representative immunoblots of p-AKT, p-PAK, and p-CrkL in B-cell progenitors from primary murine B-ALL BM cells. β-actin expression analysis was used as a loading control. (B) Representative immunoblots of Bax, Bak, Puma, Bim (EL, L, and S isoforms are identified), and Bik in p190-BCR-ABL⁺ B-cell progenitors. Cleaved caspase-3 expression was analyzed to confirm proapoptotic status of analyzed cells and β-actin was used as a loading control. (C) Level of expression and phosphorylation of Bad in WT and Vav3^−/− leukemic B-cell progenitors. (D) Representative immunoblots of Bcl-xL and Bcl-2 in p190-BCR-ABL⁺ WT or Vav3^−/− B-ALL murine BM cells. β-actin was used as a loading control (n = 3 independent experiments). (E) Normalized expression levels of proteins presented in panels B and C (n = 3 independent experiments). Vav3-deficient, p190-BCR-ABL⁺ primary B-ALL BM cells (solid bars) were normalized to their WT counterparts (empty bars). Results are shown as means ± SD. *P < .05.