Phospholipid-mediated rFVIIa action functions independently from the TF-dependent mechanism. (A) Dependence of thrombin peak heights on phospholipid concentration in the presence of 50, 25, or 6nM rFVIIa with or without FVII (closed symbols indicate 0nM FVII; open symbols, 100nM FVII). Data shown are from averaged duplicate wells. Experiments were carried out in the presence of TF (1pM; Innovin). (B) Data from panel A are represented as the percentage decrease in thrombin peak value of FVII-supplemented plasma compared with FVII-free plasma. Decrease in thrombin peak caused by the presence of FVII depends on rFVIIa concentration regardless of the phospholipid concentration. (C) Theoretical simulation of the inhibitory effect of FVII on FXa production in the presence of phospholipids.