Distribution of FAM-labeled anti–miR-155 in mice. Balb/c mice were dosed with a single tail-vein injection of 25 mg/kg of FAM-labeled anti–miR-155 or saline. (A) Distribution of FAM-labeled anti–miR-155 in cells from mice calvarium BM shown by live in vivo confocal microscopy imaging. Blood vessels are shown by Evans blue staining in red and FAM-labeled anti–miR-155 in green. (B) Distribution of FAM-labeled anti–miR-155 in cells from mice organs shown by immunofluorescent imaging. Cell nuclei are shown by DAPI staining in blue and FAM-labeled anti–miR-155 in green. Scale bar indicates 100 μm. (C) Distribution of FAM-labeled anti–miR-155 in BM, spleen, and liver as measured by flow cytometry. Statistical analysis of flow cytometry results is shown in the lower panel in bar graph. Data are shown as means ± SD. **P < .01. (D) Seven SCID mice were dosed with a single tail-vein injection of 25 mg/kg of FAM-labeled anti–miR-155 or saline immediately after injection of mCherry-Luc⁺-BCWM1 cells. Distribution of FAM-labeled anti–miR-155 in cells from the organs of mice was detected by immunofluorescent imaging and data from 3 representative mice are shown. Cell nuclei are shown by DAPI staining (blue), mCherry-Luc⁺-BCWM1 cells in red, and FAM-labeled anti–miR-155 in green. Scale bar indicates 100 μm.