Anti-Ly6G does not change LTB₄ receptor sensitivity or LTB₄-induced Ca²⁺ flux. (A) Purified neutrophils were loaded with Fura 2AM for 30 minutes at 37°C. Cells were then washed and pretreated with isotype or anti-Ly6G (10 μg for 10 minutes), and the Ca²⁺ concentration was monitored continuously with a Hitachi F-4500 calcium fluorometer from 0-200 seconds. LTB₄ at 100, 1, or 0.1nM was added 50 seconds after reading. Shown is the isotype (Iso) and anti-Ly6G overlap. (B) BM cells were pretreated with isotype or anti-Ly6G (10 μg for 10 minutes) and neutrophil migration toward LTB₄ at a range of concentrations was evaluated after 3 hours using flow cytometry. Results are representative of 2 independent experiments. *P < .05.