Coordinated expression of HLA-DQ and HLA-DOβ in professional APCs. (A) The mean fluorescence intensity (MFI) of flow cytometric staining for HLA-DR (left) and HLA-DQ (right) are shown for primary B cells (CD19⁺), monocytes (CD14⁺), T cells (CD3⁺), hematopoietic stem cells (CD34⁺), immature and mature dendritic cells (DCs), MJS melanoma cells with and without transduced HLA-DQ and HLA-DO, EBV-transformed B-cell lines (EBV-LCL) from patient MRJ, as well as fibroblasts with and without IFN-γ treatment. For primary cells means and SDs of duplicate stainings are shown. (B) mRNA expression of invariant chain (Ii), DMα and DMβ, and DOα and DOβ was measured by microarray gene expression analysis in primary B cells, monocytes, T cells, hematopoietic stem cells (CD34⁺), immature and mature DCs, MJS melanoma cells, EBV-LCL from patient MRJ, as well as fibroblasts with and without IFN-γ treatment. Relative gene expression is depicted for the mean of 3 independent samples for each cell type.