Figure 2
Kinetic analysis of mutant ZPI reactions with factor Xa in the absence and presence of cofactors. (A) Progress curves for reactions of 480nM wild-type and mutant ZPIs as indicated with 3nM factor Xa in the absence of cofactors. (B) Progress curves for reactions of 16nM wild-type and mutant ZPIs as indicated with 0.25nM factor Xa in the presence of 13nM PZ, 25μM phospholipid, and 5mM Ca2+. Solid lines are fits by a single exponential function.
