Figure 1
Figure 1. Expression of endosialin in the postnatal mouse retina. Whole-mount retinas from P2, P3, and P4 wild-type 129/SvJ mice were stained as follows. (A) Anti-endosialin or anti-NG2 polyclonal Ab followed by Alexa Fluor 555 anti–rabbit Ig. Scale bar indicates 250 μm. (B) Anti-endosialin or anti-NG2 polyclonal Ab and Alexa Fluor 555 anti–rabbit Ig (red), FITC–isolectin B4 (green), and DAPI (blue). Scale bar indicates 50 μm. (C) Anti-endosialin mAb 3K2L (supplemental Figure 2) and anti–rat Ig (green), anti-NG2 and anti–rabbit Ig (red), FITC–isolectin B4 (white), and DAPI (blue). Scale bar 50 μm. (D) Anti-endosialin polyclonal Ab followed by Alexa Fluor 555–anti–rabbit Ig (red), FITC–isolectin B4 (green), and DAPI (blue). Endosialin expression is detected on pericytes (arrowheads) but not endothelial cells (arrows). Scale bar indicates 25 μm.

Expression of endosialin in the postnatal mouse retina. Whole-mount retinas from P2, P3, and P4 wild-type 129/SvJ mice were stained as follows. (A) Anti-endosialin or anti-NG2 polyclonal Ab followed by Alexa Fluor 555 anti–rabbit Ig. Scale bar indicates 250 μm. (B) Anti-endosialin or anti-NG2 polyclonal Ab and Alexa Fluor 555 anti–rabbit Ig (red), FITC–isolectin B4 (green), and DAPI (blue). Scale bar indicates 50 μm. (C) Anti-endosialin mAb 3K2L (supplemental Figure 2) and anti–rat Ig (green), anti-NG2 and anti–rabbit Ig (red), FITC–isolectin B4 (white), and DAPI (blue). Scale bar 50 μm. (D) Anti-endosialin polyclonal Ab followed by Alexa Fluor 555–anti–rabbit Ig (red), FITC–isolectin B4 (green), and DAPI (blue). Endosialin expression is detected on pericytes (arrowheads) but not endothelial cells (arrows). Scale bar indicates 25 μm.

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