Human CD45⁺ PBMC and platelet reconstitution in humanized mice. Sublethally irradiated NOD/SCID/γc^−/− (NSG; n = 14) and NOD/SCID (n = 28) mice were transplanted with human CD34⁺ fetal liver cells alone or along with human fetal thymic tissue, and the levels of human CD45⁺ PBMCs and platelets in blood were analyzed between 9 and 18 weeks after human cell/tissue transplantation. (A) Percentage of human CD45⁺ cells in PBMCs and of human CD42a⁺ platelets in peripheral blood. Each symbol represents an individual mouse. (B) Flow cytometry profiles of humanized mouse PBMCs stained with anti–human CD45 and anti–mouse CD45, and platelets stained with anti–human platelet marker CD42a and anti–mouse platelet marker CD41. (C) Representative flow cytometry profiles of bone marrow cells from humanized mice stained with anti–human CD45 and CD61 (right), or with isotype control monoclonal antibodies (left). (D) Immunohistologic staining of bone marrow sections with anti–human CD61. Shown are samples from representative control (left) and humanized (right) mice.