Differential regulation of KLF13 protein in human and mouse T lymphocytes. (A-B) Western blot analysis of KLF13 expression in mouse (A) or human (B) T cells activated with anti-CD3 and anti-CD28 Abs. (C-D) RT-PCR of KLF13 mRNA levels in mouse (C) and human (D) T cells activated with anti-CD3 and anti-CD28 Abs. (E-F) Western blot analysis of KLF13 expression in human (E) or mouse (F) T cells treated with DMSO (vehicle control), MG-132 (5 μg/mL) or lactacystin (10μM). Relative intensity was determined by ImageJ Version 1.46 software (National Institutes of Health). Data are representative of 3-7 similar experiments. IB indicates immunoblot. Data are mean ± SD.